Abstract
We developed a biomineral-inspired hybrid material composed of CaCO3 and an organic polymer as a column packing material for HPLC. This material combines a hierarchical mesoporous structure and the functionality of the polymer. The surface of monodispersed mesoporous CaCO3 microspheres was modified with poly(maleic acid-alt-1-octadecene) (PMAcO) comprising hydrophobic alkyl chains and anionic carboxylate groups. PMAcO adsorbed onto the surface of CaCO3 through electrostatic interaction between Ca2+ sites and carboxylate groups, resulting in an octadecene coated microsphere interface. These microspheres were applied as a HPLC column and exhibited reversed-phase retention behavior in the separation of alkylbenzenes. This column showed high alkaline mobile phase resistance compared with the conventionally applied ODS column packing material. Quantitative analysis of the basic antidepressants clomipramine and imipramine spiked into whole blood was achieved with an alkaline mobile phase, demonstrating the potential of the biomineral-inspired material as a HPLC stationary phase for practical applications in routine analyses of basic drugs requiring alkaline mobile phases.
Highlights
Biominerals are inorganic–organic hybrid materials with hierarchically organized structures from nanoscopic to macroscopic scales.[1]
Preparation and characterization of amphiphilic polymer modified monodisperse CaCO3 microspheres Bare monodisperse mesoporous CaCO3 microspheres were prepared by a simple mixing method of two solutions at room temperature according to the literature.[13]
poly(maleic acid-alt-1-octadecene) (PMAcO) was obtained by the hydrolysis of repeating maleic anhydride units in poly(maleic anhydride-alt-1-octadecene) (PMAO), as shown in Scheme 1.20 Hydrolysis was confirmed by FT-IR spectra (Fig. S1, Electronic supplementary information (ESI)†), in which the peak at 1780 cmÀ1 derived from maleic anhydride decreased, while the peak at 1729 cmÀ1 derived from maleic acid was enhanced
Summary
Biominerals are inorganic–organic hybrid materials with hierarchically organized structures from nanoscopic to macroscopic scales.[1]. A biomimetic hybrid material consisting of CaCO3 mesoporous microspheres and an alternating copolymer for reversed-phase HPLC Inspired by CaCO3 based biominerals like eggshells, alternating amphiphilic copolymer-modified monodisperse mesoporous CaCO3 microspheres were applied as a HPLC stationary phase for analyses of basic drugs requiring alkaline mobile phases.
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