968 PROGNOSTIC FACTOR OF CIRCULATING TUMOR CELLS IN HIGH RISK NON-MUSCLE INVASIVE BLADDER CANCER

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research II1 Apr 2010968 PROGNOSTIC FACTOR OF CIRCULATING TUMOR CELLS IN HIGH RISK NON-MUSCLE INVASIVE BLADDER CANCER Ettore De Berardinis, Gian Maria Busetto, Alessandro Sciarra, Cristiano Cristini, Francesco Minisola, Giovanni Di Pierro, Arianna Petracca, Chiara Nicolazzo, and Paola Gazzaniga Ettore De BerardinisEttore De Berardinis More articles by this author , Gian Maria BusettoGian Maria Busetto More articles by this author , Alessandro SciarraAlessandro Sciarra More articles by this author , Cristiano CristiniCristiano Cristini More articles by this author , Francesco MinisolaFrancesco Minisola More articles by this author , Giovanni Di PierroGiovanni Di Pierro More articles by this author , Arianna PetraccaArianna Petracca More articles by this author , Chiara NicolazzoChiara Nicolazzo More articles by this author , and Paola GazzanigaPaola Gazzaniga More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1915AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The prognosis of T1G3 bladder cancer is highly variable and not predictable basing upon clinical and pathological prognostic factors. There is need for improvement in risk stratification in this population; understanding the molecular profile of individual patients could provide a more personalized and tailored treatment. Main objective was to evaluate the prognostic significance of Survivin in tumor tissues and that of Survivin expressing circulating tumor cells (CTCs) in T1G3 tumors. METHODS 108 patients with T1G3 non muscle invasive bladder cancer (NMIBC) were enrolled. Additional inclusion criteria were: tumor size<3cm; absence of CIS and multifocality. Planned follow up was 24 mo. Survivin was evaluated by RT-PCR in tumoral tissues. CTCs were isolated from blood by CELLection™ Dynabeads coated with the monoclonal antibody towards the human Epithelial Cell Adhesion Molecule. Cells were lysed and Dynabeads Oligo(dT) was used to capture poly A+ mRNA. cDNA was synthesised and analysed for the expression of CD45, CK8 and Survivin. The primary end point was disease free survival (DFS); the favourable group at 24 mo was defined as that without any clinical evidence of disease (NED); the unfavourable group was that with evidence of recurrent disease (RD) or progressive disease (PD). Tumoral Survivin expression and presence of CTC were correlated to DFS. Multivariate analysis was used to investigate whether CTC presence was independent indicator of DFS. RESULTS Survivin was found in 50% of tumors. Survivin - patients showed a longer DFS than Survivin + (÷2: 4.572; p =0.029). CTCs were found in 48/108 patients (44%); 92% of CTC were Survivin expressing. The difference in DFS between CTC - and CTC + patients was statistically significant (÷2: 28.098; p <0.001). CTC presence was found an independent prognostic factor of DFS (p<0.001). CONCLUSIONS CTC presence is an independent prognostic factor in high risk NMIBC patients. Roma, Italy© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e376 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Ettore De Berardinis More articles by this author Gian Maria Busetto More articles by this author Alessandro Sciarra More articles by this author Cristiano Cristini More articles by this author Francesco Minisola More articles by this author Giovanni Di Pierro More articles by this author Arianna Petracca More articles by this author Chiara Nicolazzo More articles by this author Paola Gazzaniga More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...