922 SURVIVIN EXPRESSING CIRCULATING TUMOR CELLS IN NON-MUSCLE INVASIVE BLADDER CANCER: THEIR IDENTIFICATION AND CHARACTERIZATION WITH CELLECTION DYNABEADS ANALYSIS

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research (II)1 Apr 2013922 SURVIVIN EXPRESSING CIRCULATING TUMOR CELLS IN NON-MUSCLE INVASIVE BLADDER CANCER: THEIR IDENTIFICATION AND CHARACTERIZATION WITH CELLECTION DYNABEADS ANALYSIS Gian Maria Busetto, Paola Gazzaniga, Chiara Nicolazzo, Arianna Petracca, Riccardo Giovannone, Vincenzo Gentile, and Ettore De Berardinis Gian Maria BusettoGian Maria Busetto Rome, Italy More articles by this author , Paola GazzanigaPaola Gazzaniga Rome, Italy More articles by this author , Chiara NicolazzoChiara Nicolazzo Rome, Italy More articles by this author , Arianna PetraccaArianna Petracca Rome, Italy More articles by this author , Riccardo GiovannoneRiccardo Giovannone Rome, Italy More articles by this author , Vincenzo GentileVincenzo Gentile Rome, Italy More articles by this author , and Ettore De BerardinisEttore De Berardinis Rome, Italy More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.499AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Prognosis of high risk non-muscle invasive bladder cancer is highly variable and unpredictable on the basis of clinical and pathological variables. Survivin, a small protein belonging to æinhibitor of apoptosisÆ family, is frequently overexpressed in bladder tumours but not detected in normal differentiated transitional epithelium. Thus, the presence of survivin transcripts in bladder tumours is considered a marker of bladder cancer progression. The evasion of apoptosis through the overexpression of survivin also represents one of the mechanisms by which tumour cells acquire the ability to enter and survive in the blood flow; consistent with this hypothesis, it was shown that survivin is often expressed by circulating tumour cells (CTCs), a subset of cancer cells that escape from the primary tumour into the bloodstream and travel through the circulation to distant sites, where are thought to possibly develop into secondary tumours. Main objective was to evaluate the presence of circulating tumor cells (CTCs) in T1G3 tumors and to characterize them by the expression of survivin. METHODS 141 patients with T1G3 non muscle invasive bladder cancer (NMIBC) were enrolled. Additional inclusion criteria were: tumor size<3cm; absence of CIS and multifocality. Planned follow up was 36 mo. Survivin was evaluated by RT-PCR in tumoral tissues. CTCs were isolated from blood by CELLection™ Dynabeads coated with the monoclonal antibody towards the human Epithelial Cell Adhesion Molecule. Cells were lysed and Dynabeads Oligo(dT) was used to capture poly A+ mRNA. cDNA was synthesised and analysed for the expression of CD45, CK8 and Survivin. The primary end point was disease free survival (DFS); the favourable group at 36 mo was defined as that without any clinical evidence of disease (NED); the unfavourable group was that with evidence of recurrent disease (RD) or progressive disease (PD). Tumoral Survivin expression and presence of CTC were correlated to DFS. RESULTS Survivin was found in 50% of tumors. Survivin - patients showed a longer DFS than Survivin + (÷2: 4.572; p =0.029). CTCs were found in 48/141 patients (34%); 92% of CTC were Survivin expressing. The difference in DFS between CTC - and CTC + patients was statistically significant (÷2: 28.098; p <0.001). CTC presence was found an independent prognostic factor of DFS (p<0.001). CONCLUSIONS CTC presence is an independent prognostic factor in high risk NMIBC patients. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e379 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Gian Maria Busetto Rome, Italy More articles by this author Paola Gazzaniga Rome, Italy More articles by this author Chiara Nicolazzo Rome, Italy More articles by this author Arianna Petracca Rome, Italy More articles by this author Riccardo Giovannone Rome, Italy More articles by this author Vincenzo Gentile Rome, Italy More articles by this author Ettore De Berardinis Rome, Italy More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...