Abstract
Ionizing Radiation (IR)-inducible promoters used as genetic switches for cancer therapy are promising tool for controlling therapeutic gene expression. Here we describe the use of a naturally secreted luciferase from the marine copepod Gaussia princeps (Gluc) for monitoring the activity and induction levels of different IR- sensitive promoters. The following promoters were tested: wild-type p53-activated fragment 1 (WAF1, 2.4 kb); early growth response factor (Egr-1, 550 bp); four tandem repeats of the transcriptional factor nuclear factor-kB (4NF-kB, 400 bp); and a combination of both Egr-1 and 4NF-kB promoters.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Paper version not known
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
