899. Glucose-Stimulated Insulin Secretion from an Engineered Human Liver Cell Line Is Regulated by ATP-Sensitive Potassium Channels and the L-Type Calcium Channel

Ann M Simpson ,Edwin Ch'ng ,Chang Tao ,Mark Lutherborrow ,Donald C Martin ,Guo Liu ,Bernard E Tuch

doi:10.1016/j.ymthe.2005.07.442

899. Glucose-Stimulated Insulin Secretion from an Engineered Human Liver Cell Line Is Regulated by ATP-Sensitive Potassium Channels and the L-Type Calcium Channel

Ann M Simpson , Edwin Ch'ng + Show 5 more

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https://doi.org/10.1016/j.ymthe.2005.07.442

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Journal: Molecular Therapy	Publication Date: May 1, 2005
License type: cc-by-nc-nd

#Artificial Beta Cell #Human Liver Cell Line Huh7 + Show 8 more

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Abstract

Introduction: One approach to the treatment of Type 1 diabetes is the creation of an 'artificial beta cell'. The stable transfection of insulin cDNA into the human liver cell line Huh7 resulted in synthesis, storage and regulated release of insulin to a glucose stimulus (Huh7ins cells). The aim of the present study was to determine if Huh7ins cells respond to glucose via activation of ATP-sensitve potassium channels (KATP) and voltage-gated calcium channels, as is the case with pancreatic beta cells.

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