890 PENILE ENHANCEMENT USING AUTOLOGOUS TISSUE ENGINEERING WITH BIODEGRADABLE SCAFFOLD: A CLINICAL AND HISTOMORPHOMETRIC STUDY

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You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research II1 Apr 2010890 PENILE ENHANCEMENT USING AUTOLOGOUS TISSUE ENGINEERING WITH BIODEGRADABLE SCAFFOLD: A CLINICAL AND HISTOMORPHOMETRIC STUDY Salvatore Sansalone, Sava V. Perovic, Rados Djinovic, Amedeo Ferlosio, Giuseppe Vespasiani, and Augusto Orlandi Salvatore SansaloneSalvatore Sansalone Rome, Italy More articles by this author , Sava V. PerovicSava V. Perovic Belgrade, Yugoslavia More articles by this author , Rados DjinovicRados Djinovic Belgrade, Yugoslavia More articles by this author , Amedeo FerlosioAmedeo Ferlosio Rome, Italy More articles by this author , Giuseppe VespasianiGiuseppe Vespasiani Rome, Italy More articles by this author , and Augusto OrlandiAugusto Orlandi Rome, Italy More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1646AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Autologous tissue engineering with biodegradable scaffolds is a new treatment option for real penile girth enhancement.The aim of this article is to evaluate tissue remodeling after penile girth enhancement using this technique METHODS Between June 2005 and May 2007, a group of 12 patients underwent repeated penile widening using biodegradable scaffolds enriched with expanded autologous scrotal dartos cells. Clinical monitoring was parallel to histological investigation of tissue remodeling. During second surgical procedure, biopsies were obtained 10–14 months after first surgery (mean 12 months, N = 6) and compared with those obtained after 22–24 months (mean 23 months, N = 6), and control biopsies from patients who underwent circumcision (N = 5). Blind evaluation of histomorphometrical and immunohistochemical finding was performed in paraffin sections.Penile girth gain in a flaccid state ranged between 1.5 and 3.8 cm (mean 2.1 _ 0.28 cm) and in full erection between 1.2 and 4 cm (mean 1.9 _ 0.28 cm). Patients' satisfaction, defined by a questionnaire, was good (25%) and very good (75% RESULTS In biopsies obtained 10–14 months after first surgery, highly vascularized loose tissue with collagen deposition associated with small foci of mild chronic and granulomatous inflammation surrounding residual amorphous material was observed. Fibroblast-like hyperplasia and small vessel neoangiogenesis occurred intimately associated with the progressive growth of vascular-like structures from accumulation of CD34 and alpha-smooth muscle actin-positive cells surrounding residual scaffold-like amorphous material. Capillary neoangiogenesis occurred inside residual amorphous material. In biopsies obtained after 22–24 months,inflammation almost disappeared and tissue closely resembled that of the dartos fascia of control group CONCLUSIONS Autologous tissue engineering using expanded scrotal dartos cells with biodegradable scaffolds is a new and promising method for penile widening that generates progressive accumulation of stable collagen-rich, highly vascularized tissue matrix that closely resemble deep dartos fascia © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e348 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Salvatore Sansalone Rome, Italy More articles by this author Sava V. Perovic Belgrade, Yugoslavia More articles by this author Rados Djinovic Belgrade, Yugoslavia More articles by this author Amedeo Ferlosio Rome, Italy More articles by this author Giuseppe Vespasiani Rome, Italy More articles by this author Augusto Orlandi Rome, Italy More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...