866 DIFFERENTIAL MIRNA EXPRESSION PROFILES IN URINES FROM BLADDER CANCER PATIENTS

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research1 Apr 2011866 DIFFERENTIAL MIRNA EXPRESSION PROFILES IN URINES FROM BLADDER CANCER PATIENTS Lourdes Mengual, Mercedes Ingelmo-Torres, Maria J. Ribal, Cristina Gazquez, Juan Jose Lozano, and Antonio Alcaraz Lourdes MengualLourdes Mengual Barcelona, Spain More articles by this author , Mercedes Ingelmo-TorresMercedes Ingelmo-Torres Barcelona, Spain More articles by this author , Maria J. RibalMaria J. Ribal Barcelona, Spain More articles by this author , Cristina GazquezCristina Gazquez Barcelona, Spain More articles by this author , Juan Jose LozanoJuan Jose Lozano Barcelona, Spain More articles by this author , and Antonio AlcarazAntonio Alcaraz Barcelona, Spain More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.690AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES To identify differentially expressed miRNAs in urine from patients with bladder cancer compared to controls and also high compared to low grade tumours. Furthermore, correlating differentially expressed miRNAs with gene expression profiles in the same patient group. METHODS Thirty urine samples from patients with urothelial cell carcinoma of the bladder (UCC) (10 Ta low grade, 10 T1 high grade and 10 ”dT2 high grade) and 10 urine samples from urological patients without malignancy were collected and processed for RNA extraction. miRNA expression patterns of the 40 urine samples were analyzed by Real Time quantitative PCR in TaqMan Arrays containing 738 miRNAs. Values from differentially expressed miRNA were correlated with gene expression values of the same patients (data obtained from a previous study). RESULTS Thirty two miRNAs were found differentially expressed between UCC patients and controls (p <0.05). Sixteen of these miRNAs were inversely correlated with 15 genes differentially expressed between urine from UCC patients and controls. In addition, 37 miRNAs were found differentially expressed between low and high grade tumours (p <0.05). Thirteen of these miRNAs were inversely correlated with 24 genes differentially expressed between high and low grade tumours. CONCLUSIONS In this initial study we show that miRNA expression profile in urine samples is able to discriminate between bladder cancer and control patients and between high and low grade tumours. We also show that miRNA expression profiles correlate with gene expression data. Further studies are needed to develop an accurate and noninvasive method for bladder cancer diagnosis based in miRNA expression patterns. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e347 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Lourdes Mengual Barcelona, Spain More articles by this author Mercedes Ingelmo-Torres Barcelona, Spain More articles by this author Maria J. Ribal Barcelona, Spain More articles by this author Cristina Gazquez Barcelona, Spain More articles by this author Juan Jose Lozano Barcelona, Spain More articles by this author Antonio Alcaraz Barcelona, Spain More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...