827 COLON-TO-BLADDER AFFERENT CROSS-SENSITIZATION TO ENHANCE BLADDER AND URETHRAL PAIN BEHAVIORS IN RATS WITH TNBS-INDUCED COLITIS

Abstract

You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Interstitial Cystitis1 Apr 2012827 COLON-TO-BLADDER AFFERENT CROSS-SENSITIZATION TO ENHANCE BLADDER AND URETHRAL PAIN BEHAVIORS IN RATS WITH TNBS-INDUCED COLITIS Satoru Yoshikawa and Naoki Yoshimura Satoru YoshikawaSatoru Yoshikawa Pittsburgh, PA More articles by this author and Naoki YoshimuraNaoki Yoshimura Pittsburgh, PA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.917AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES There is a significant overlap in chronic pelvic pain syndrome in women, which includes interstitial cystitis/bladder pain syndrome (IC/BPS), irritable bowel syndrome (IBS) and endometriosis. Previous animal studies also demonstrated that experimental colitis leads to the electrophysiological hyperexcitability of afferent neurons innervating the bladder, resulting in frequent voiding in rats. However, it is not directly examined whether colon irritation increases pain sensation in the lower urinary tract. This study therefore investigated whether pain behavior in response to bladder and urethral irritation is enhanced in rats with experimental colitis. METHODS In female SD rats, experimental colitis was induced by trinitrobenzene sulfonic acid (TNBS, 50mg/mL in 50% ethanol, 0.4mL) through a transanally placed catheter. In some rats, capsaicin was administered subcutaneously for 2 consecutive days (total: 125 mg/kg) starting 6 days before behavioral studies to desensitize capsaicin-sensitive C-fiber afferent pathways. At 10 days after TNBS application, resiniferatoxin (RTx, 0.3μM) was instilled into the bladder for 1 min via a urethral catheter, and then two types of nociceptive behaviors such as licking (lower abdominal licking; pudendal nerve-dependent urethral pain behavior) and freezing (motionless head turning; pelvic nerve-dependent bladder pain behavior) were scored every 5sec for 15min. RESULTS In TNBS-treated rats, intravesical application of RTx showed significantly increased licking behavior with a peak in the early phase (0-5 min) (p<0.001) and freezing behavior with a peak in the late phase (10-15min) (p<0.001) compared to vehicle-treated rats, in which RTX application induced minimal changes in pain behavior (Fig). C-fiber desensitization by capsaicin pretreatment dramatically and significantly suppressed these enhanced pain behaviors (p<0.001 and p<0.001, respectively). CONCLUSIONS These results indicate that colitis (10 days) enhances pain sensation (i.e., hyperalgesia) derived from the bladder and urethra due to colon-to-bladder afferent cross-sensitization via activation of C-fiber afferent pathways. These mechanisms might contribute to overlapped pain symptoms in IC/BPS and IBS. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e338 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Satoru Yoshikawa Pittsburgh, PA More articles by this author Naoki Yoshimura Pittsburgh, PA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...