8-Hydroxyquinoline-5-Sulfonic Acid-Containing Poly(Vinyl Alcohol)/Chitosan Electrospun Materials and Their Cu2+ and Fe3+ Complexes: Preparation, Antibacterial, Antifungal and Antitumor Activities.

Milena Ignatova,Nevena Manolova,Iliya Rashkov,Rositsa Kukeva,Ani Georgieva,Reneta Toshkova,Radostina Stoyanova,Nadya Markova

doi:10.3390/polym13162690

Abstract

Novel poly(vinyl alcohol) (PVA)/chitosan (Ch)-based fibrous materials containing an ionizable model drug, 8-hydroxyquinoline-5-sulfonic acid (SQ), were successfully fabricated by electrospinning. Complexes between the components of the crosslinked PVA/Ch/SQ mats and Cu2+ and Fe3+ ions were formed. The coordination of these ions in the mats was examined by electron paramagnetic resonance spectroscopy (EPR). The microbiological screening against S. aureus and C. albicans revealed that both the incorporation of SQ in the mats and the complexation with Cu2+ and Fe3+ imparted to these materials antibacterial and antifungal activities. Moreover, the SQ-containing mats and their complexes displayed good cytotoxicity against human cervical HeLa tumor cells. The most prominent was the cytotoxicity of the Cu2+ complex of the mats. The combined antibacterial, antifungal and in vitro antitumor activities render these novel materials promising candidates for wound dressing applications and for application in the local treatment of cervical tumors.

Highlights

Electrospinning is a cutting-edge technology for producing continuous polymer fibers with diameters ranging from a few nanometers to several micrometers [1,2]
Evaluation of the interaction between S. aureus 3359 and crPVA, cr(PVA/Ch), cr(PVA/ Ch)/SQ and Fe3+(Cu2+) complex of cr(PVA/Ch)/SQ mats was performed by SEM observation with Jeol JSM-5510 (Jeol Ltd., Tokyo, Japan) of S. aureus cells adhered to the mat surface that had been in contact with a bacteria suspension
Fe3+ complexes against Gram-positive bacteria S. aureus and fungi C. albicans were estimated by performing microbiological tests consisting of determining the diameter of the zone of inhibition around the fibrous materials

Summary

Introduction

Electrospinning is a cutting-edge technology for producing continuous polymer fibers with diameters ranging from a few nanometers to several micrometers [1,2]. Electrospun micro- and nanofibrous materials have been considered as carriers of low-molecular-weight compounds with antimicrobial and antitumor properties [3,4]. This is due to some important characteristics of the fibrous materials, such as their high specific surface area and highly porous structure [5]. PVA was selected for incorporation into fibrous materials intended for biomedical purposes since this polymer is biocompatible and has low toxicity and functional reactive groups. There are a limited number of reports on the preparation of electrospun materials from mixed PVA/Ch containing drugs such as antibiotics and antitumor drugs [24,25,26,27,28,29] or biologically active compounds of natural origin (curcumin) [30]

Methods

Results

Conclusion