Abstract
Functional insertion of activated src or PyMT oncogenes induced the tumorigenic potential of PC AA/C1 cells in athymic mice. Transfer of the Py early region genes alone or combined with c-src shortened the latency period and induced highly progressive tumors. PC cells transfected by mutated src induced exhibited elevated pp60<sup>src</sup> tyrosine kinase activity, HGF-dependent invasion in collagen gels and overexpression of MET by Northern and Western· blots. Expression of the HGF gene was detected by RT-PCR and Southern blot in parental and oncogene-transfected cells. Our results indicate that activation of the src tyrosine kinase, an early event during human colon cancer progression, may be involved: (1) in the adenoma-carcinoma conversion, (2) activation of the invasion and metastatic cascades under the control of HGF and MET.
Published Version
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