Abstract

The real-time spatiotemporal analysis of thrombus formation is still difficult in blood pumps. Platelets are activated by blood flow and pump materials. Activated platelets release ADP, ATP, serotonin, etc. ATP can be detected using a luciferin-luciferase (L-L) reagent with high sensitivity. A L-L reagent reacts with ATP to emit light, then it can be detected with a photomultiplier tube (PMT) and a CCD camera, and this would realize the detailed analysis of platelet activation and also the thrombus formation in blood pumps. As a preliminary study, the blood clotting in the test tube was observed using a L-L reagent with a PMT and an electron multiplying CCD camera.

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