Abstract
An apparatus interfaced with a microcomputer was constructed to maintain dissolved oxygen concentration (DO) at a constant level during cultivation. Air and oxygen flow rates and agitation speed were automatically varied by the instructions stored in the microcomputer. A program was written to keep the DO level constant during the cultivation for the cases of slow DO change due to unbalance of oxygen transfer rate and abrupt DO changes due to noise such as attachment of air bubbles to the DO sensor, addition of antifoam and deficiency of a carbon source. No manual operation was needed to change the preset values and it was possible to maintain DO level at 2 ppm for the cultivation of Candida brassicae and obtain high density biomass (53 g/L) after 9 hrs. This DO-stat system could be used for cultivation of microorganisms with various specific oxygen uptake rates without modification of the program. It was also possible to keep running costs to a minumum by optimum choice of flow rate and agitation speed.
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