Abstract
Publisher Summary Electrophoresis has proven to be a powerful resolving technique for both analytical and preparative biochemical experiments. Electrophoretic resolution of ribosome and polysome components was successfully achieved. This chapter discusses the following topics: (1) one-dimensional electrophoretic resolution of nucleosomes, (2) enzymatic preparation of chromatin and chromatin fractionation, (3) two-dimensional electrophoretic resolution of DNA and detection of specific sequences, (4) two-dimensional electrophoretic resolution of proteins using Triton-acid-urea gels, and (5) further applications for electrophoretic analyses. This chapter also have described techniques to (1) determine the overall electrophoretie patterns of various nucleoprotein species, (2) analyze the bulk DNA lengths of such species by performing a second dimension of electrophoresis in the presence of SDS, (3) determine the position of specific sequences in such two-dimensional displays after DNA transfer and nucleic acid hybridization, and (4) analyze the bulk protein compositions of such species by performing a second dimension of electrophoresis in Triton-acidurea gels.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
