Abstract
Neem is considered to be one of the most promising plants for producing pesticides, pharmaceutical, as well as many commonplace materials. A protocol for shoot formation from nodal and stem explants is described. Stem nodes and stem segments were obtained from mature tree and cultured in Murashige and Skoog medium (MS) supplemented with 0.5 μM thidiazuron (TDZ), and 0.5 uM naphthaleneacetic acid (NAA). Stem node explants produced multiple shoots which were separated and cultured on MS supplemented with 0.01, 0.03, 0.5, or 0.9 uM TDZ with 0.5 uM NAA. Stem explants produced callus which regenerated shoots upon transfer to a fresh medium. Formed shoots produced roots in proliferation medium or rooted in MS supplemented with 3.3 uN indolebutyric acid, and were transferred to soil. Number of produced shoots increased with increasing TDZ concentration but shoot and root length decreased.
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