Abstract
that hypermutation play an important role during diversification and adaptation of Achromobacter sp. Methods: The mutation frequency of Achromobacter sp. isolates is estimated by plating isolates onto LB agar and LB agar containing rifampicin. Mutation frequency values are reported as the number of rifampicin-resistant colonies in proportion to the total viable count. Genes of the MMR-system of Achromobacter will be amplified by PCR and subsequently sequenced. Results: In this study we investigate the prevalence of hypermutator strains of Achromobacter sp. among patients affiliated with the CF Centre at Aarhus University Hospital. MMR gene sequences of potential hypermutators and normomutators will be compared in order to decipher the genetics of hypermutation in Achromobacter sp. Conclusion: Hypermutation might be a key element of genetic adaptation of Achromobacter sp. in the CF lung. Adaptation of Achromobacter sp. to changing environments is essential for persistence in the CF lung.
Published Version
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