#55 : Ooplasm Diameter as a Predictor for Pre-Implantation Development in Intracytoplasmic Sperm Injection Cycles

Abstract

Background and Aims: The use of noninvasive measures such as ooplasm diameter (OD) for oocyte evaluation to predict the results of intracytoplasmic sperm injection (ICSI) is of great clinical importance. In this study, we determine whether OD is a significant predictor for pre-implantation development in intracytoplasmic sperm injection cycles in order to select good quality oocytes. Method: A total of 1,130 treatment cycles from 1,130 women who underwent oocyte retrieval during mild stimulation cycles between February 2018 to June 2019 were retrospectively reviewed. The correlation of OD with embryonic outcomes was analyzed. Results: The logistic regression analysis demonstrated that the OD was not correlated with embryonic outcomes. However, our preliminary analysis demonstrated that the embryonic outcomes in the oocytes with small or large OD tended to be lower than those oocytes with optimal OD. Therefore, we stratified the oocytes into six groups according to the OD using statistical software (Group A, OD < 110.19; B 110.19 [Formula: see text] OD < 112.22; C, 112.22 [Formula: see text] OD < 113.67; D, 113.67 [Formula: see text] OD < 115.01; E, 115.01 [Formula: see text] OD < 116.02; F, 116.02 [Formula: see text] OD). The highest blastocyst utilization rate was observed in Group D. The blastocyst utilization rate in Group A (the smallest-diameter group) and F (the largest-diameter group) were significantly lower than that in Group C and D. Conclusion: The optimal range of OD is associated with the higher blastocyst utilization rate after ICSI. These results suggest that OD might be used as a predictive parameter to select/de-select the oocytes for insemination, leading to better control of the quantity and quality of the embryos obtained, reducing to a minimum the ethical issues related to excess embryos.