Abstract
This chapter presents a procedure for the preparation of coenzyme A- glutathione disulfide and mixed disulfides. For coenzyme A- glutathione disulfide, the thiolsulfonate analog of glutathione disulfide (GSSO 2 G) is prepared and allowed to react with the more expensive coenzyme A. In the quantitative analysis of mixed disulfides, every mixed disulfide presents its individual possibilities and problems in analyses. Various chromatographic and electrophoretic methods can be quantitated for known compounds. In some cases, the reduction of the disulfide may yield a thiol that can accurately and specifically be determined quantitatively, e.g., by enzymatic analysis. A general method for the determination of the titer of disulfide bonds has been based on the reduction of the disulfide, with GSH monitored spectrophotometrically at 340 nm by coupling with the reaction catalyzed by glutathione reductase.
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