Abstract
Publisher Summary The development of recombinant DNA technology has also made important contributions to gene mapping, especially by combining cell hybrids and cloned gene probes for chromosomal and regional assignment of many structural genes that may not be expressed in cultured cells or cell hybrids. This chapter discusses the use of somatic cell hybrids in apolipoprotein gene mapping. Although many assays can be performed to identify particular genetic markers that have been assigned to particular human chromosomes, the following two analyses are most commonly and conveniently used, especially when large numbers of cell hybrids will have to be analyzed for each of the 24 different human chromosomes. The chapter indicates that DNA sequence markers can be effectively used for fine structure mapping of specific regions of the chromosome in which various deletions produced by chromosomal breaks and localized in a small region may not be resolvable by current cytogenetic or in situ hybridization techniques.
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