Abstract
In this chapter, we report the fundamental criteria and applications of two-dimensional correlation spectroscopy (2DCOS) in the environmental studies of the natural organic matter (NOM) and all its fractions like dissolved organic matter (DOM) and humic substances. The applications of the 2DCOS techniques to spectroscopic and chromatographic signals offer many peculiar advantages. 2DCOS allows to describe the dynamic evolutions, chemical reactions, and structural changes in the complex molecular systems of NOM exploiting the changes in correlations among the functional groups. The transition from the conventional mono-dimensional examination of spectra to the examination of 2DCOS spectra allows to improve the analytical resolution of data supporting a better interpretation of the chemical information present. In addition, 2DCOS plots also allow to describe the sequential order of the structural changes occurring during the processes of aggregation and degradation of NOM and during the evolution of other processes like interaction with pollutants and xenobiotics.
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