Abstract
Droplet-based bioprinting (DBB) offers greater advantages due to its simplicity and agility with precise control on deposition of biologics including cells, growth factors, genes, drugs, and biomaterials, and has been a prominent technology in the bioprinting community. Due to its immense versatility, DBB technology has been adopted by various application areas, including, but not limited to, tissue engineering and regenerative medicine, transplantation and clinics, pharmaceutics and high-throughput screening, and cancer research. Despite the great benefits, the technology currently faces several challenges such as a narrow range of available bioink materials, bioprinting-induced cell damage at substantial levels, limited mechanical and structural integrity of bioprinted constructs, and restrictions on the size of constructs due to lack of vascularization and porosity. This chapter presents DBB technology and its modalities including inkjet, electrohydrodynamic, acoustic, and microvalve bioprinting. The recent notable studies are highlighted and the relevant bioink and substrate biomaterials are expounded. In addition, current limitations are discussed and future prospects are presented to the reader.
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