Abstract
<h2>Abstract</h2> Recent advances in microscopy enable three-dimensional live imaging at a high resolution. Long-term live imaging of a multicellular organism requires immobilization of the organism under stable physiological conditions. Despite proper immobilization, challenges remain within live imaging data analysis due to other intrinsic and extrinsic dynamics, which can result in misalignments of an image series over time. 4Dia, an ImageJ/Fiji macro script, aligns 3D timelapse images through Z-stacks as well as over time using any user selected channel. 4Dia can be used for essentially any tissue sample with no limit on the size of Z-stack or the number of timepoints.
Highlights
Recent advances in microscopy enable three-dimensional live imaging at a high resolution
Current code version Permanent link to code/repository used for this code version Permanent link to reproducible capsule Legal code license Code versioning system used Software code languages, tools and services used Compilation requirements, operating environments and dependencies
Cial tool that is used daily in various subfields of biology, including cell high temporal resolution is crucial for live imaging, especially for biology and genetics
Summary
Recent advances in microscopy enable three-dimensional live imaging at a high resolution. V1.1 https://github.com/SoftwareImpacts/SIMPAC- 2021- 157 N/A MIT License None IJM (ImageJ Macro language) Fiji (ImageJ v1.53f), ‘MultiStackReg’ plugin, and ‘TurboReg’ plugin N/A chlee@albany.edu Since the advent of optical microscopy, imaging has become a cru- fixed samples, where the temporal factor can be ignored.
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