Abstract
Introduction: Administration of epinephrine (Epi) during cardiopulmonary resuscitation (CPR) might be associated with deleterious adverse effects. The aim of the present study was to assess whether vasopressin (Vaso) or the combination of Epi and Vaso can suppress the expression of NF-κB in rat’s hippocampus compared with Epi alone in a rat model of cardiac arrest (CA) after asphyxia. Methods: The rat model of cardiac arrest (CA) after asphyxia was used in this study. 200 rats were divided into 5 groups randomly (n=40 rats in each group) Sham-operated group: no asphyxia and no CPR, only for endotracheal intubation. Control group: after CA, CPR was performed immediately and 1 ml of saline was administered. Epi group: CPR was implemented after CA and 1 ml of Epi (0.2mg/kg) was administered. Vaso group: after CA, CPR was carried out and 1 ml of Vaso (0.8U/Kg) was injected. Combination group: CPR was performed after CA. 1 ml of Epi (0.2mg/kg) and 1 ml of Vaso (0.8U/Kg) were administered. The rats in control group exhibited the lowest survival rate and therefore were abandoned in the following experiment. The rats survived from other groups were killed in 1, 3, 6 and 12 hours after CPR, respectively, and the brains were taken out. We tested the expression of NF-κB in rat’s hippocampus by immunohistochemistry. The restoration of spontaneous circulation (ROSC) rate was recorded and the pathological changes of the brains were observed in each group. Results: Compared to Epi group, the expressions of NF-κB in Vaso group and combination group were lower (P<0.05). No significant difference was seen between Vaso group and combination group (P>0.05). HE staining demonstrated there was no difference in morphology changes among Epi, Vaso and combination group under light microscope. Compared to Sham-operated group, the edema of neurons in Hippocampus was seen in the other three groups. The damage of Mitochondria was more serious in Epi group than in Vaso and combination group under electron microscope. No difference of Mitochondria damage was seen between Vaso and combination group. Conclusions: Vaso can alleviate cerebral ischemia reperfusion injury during CPR and suppress the expression of NF-κB in Hippocampus of rats with CA after asphyxia.
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