Abstract
Introduction: Epinephrine (Epi) is recommended in cardiopulmonary resuscitation (CPR). Vasopressin (Vaso) has been studied for use in CPR as an alternative. Methods: The rat model of cardiac arrest (CA) after asphyxia was used in the current study. 200 rats were divided into 5 groups randomly (n=40 rats in each group) Sham-operated group: no asphyxia and no CPR, only for endotracheal intubation. Control group: after CA, CPR was performed immediately and 1 ml of saline was administered. Epi group: CPR was implemented after CA and 1 ml of Epi (0.2mg/kg) was administered. Vaso group: after CA, CPR was carried out and 1 ml of Vaso (0.8U/Kg) was injected. Combination group: CPR was performed after CA. 1 ml of Epi (0.2mg/kg) and 1 ml of Vaso (0.8U/Kg) were administered. The rats in control group exhibited the lowest survival rate and therefore were abandoned in the following experiment. The rats survived from other groups were killed in 1, 3, 6 and 12 hours after CPR, respectively, and the brains were taken out. We tested the expression of Caspase-3 in rat’s hippocampus by immunohistochemistry. The restoration of spontaneous circulation (ROSC) rate was recorded and the pathological changes of the brains were observed in each group. Results: Compared to Epi group, the expressions of Caspase-3 in Vaso group and combination group were lower (P<0.05). No significant difference was seen between Vaso group and combination group (P>0.05). HE staining demonstrated there was no difference in morphology changes among Epi, Vaso and combination group under light microscope. Compared to Sham-operated group, the edema of neurons in Hippocampus was seen in the other three groups. The damage of Mitochondria was more serious in Epi group than in Vaso and combination group under electron microscope. No difference of Mitochondria damage was seen between Vaso and combination group. Conclusions: Vaso can reduce the expression of Caspase-3 in Hippocampus of rats with CA after asphyxia and alleviate the ischemia reperfusion injury during CPR.
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