Abstract
Publisher Summary This chapter focuses on determination of pterins by liquid chromatography/electrochemistry. Pterins are of interest because of their possible involvement in a number of diseases. Pterins are obtained from the sources such as pterin, pterin-6-carboxylic acid, 6-methylpterin, and xanthopterin. A property of the pterins essential to their biological role as enzyme cofactors is their electrochemistry. Fully oxidized pterins can be reversibly reduced to 5,8-dihydropterins which rapidly tautomerize to 7,8-dihydropterins. These 7,8-dihydropterins can either be further reduced to 5,6,7,8-tetrahydropterins or be oxidized back to the fully oxidized state. Dual-electrode detector uses two working electrodes placed adjacent to each other and normal to the direction of flow—that is, in a parallel-adjacent configuration. Using this detector, one electrode is operated at an oxidizing potential to detect the dihydro- and tetrahydropterins while the other electrode is operated at a reducing potential to detect the oxidized pterins, thus the three stable oxidation states of the pterins can be detected simultaneously. For the determination of pterins in urine, a 25-cm chromatography column thermostatted at 30° is used. The mobile phase contains 5% methanol (v/v). A flow rate of 1.0 ml/min is employed.
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