452 - Sciatic Nerve Ligation Causes Impairment of Mitochondria Associated with Changes in Distribution, Respiration and Cardiolipin Composition in Related Spinal Cord Neurons in Rats

Abstract

Sciatic nerve irritation is often associated with disturbed Ca 2+ homeostasis in related neurons of the spinal cord. Since mitochondria substantially contribute to Ca 2+ homeostasis and little information is available we studied effects of loose sciatic nerve ligation, a chronic constriction injury (CCI), on neuronal mitochonderia of the L3-L6 region. Three groups of rats (untreated, sham operated, and ligated) were explored. For the characterization of mitochondria, specimens of the L3-L6 spinal cord region were evaluated with respect to intracellular localization using pyruvate dehydrogenase immunohistochemistry and Mitotracker Red, and the ATP producing machinery using LC-MS/MS technique for the analysis of cardiolipin and high-resolution respirometry for the measurement of oxygen consumption. Thereby, the phospholipid cardiolipin supports electron transfer within the respiratory chain as part of mitochondrial respiration and is of high impact on the physical properties of the mitochondrial membrane system. Histological analysis of spinal cord motor neurons revealed clustering of mitochondria in ipsilateral samples from ligated animals 14 d after the insult. This phenomenon was similarly evident in the respective contralateral side. The intensity of MT-Red staining was enhanced exclusively at the ipsilateral side indicating increased mitochondrial activity. CCI of the sciatic nerve caused massive changes in the composition of cardiolipin reflecting mitochondrial impairment in the early phase followed by regeneration processes as late response. Sciatic nerve CCI caused decrease in the capacity of mitochondrial ATP production that recovered within 14 d after treatment. In conclusion we provide evidence that clustering of mitochondria, already verified for the spinal cord sensory neurons after CCI, also occurs in the respective motor neurons. Further we have demonstrated transient impairment of the capacity of mitochondrial ATP production in tissue samples. Stress-dependent changes in cardiolipin composition are sensitive markers and mediators of the response process including impairment and regeneration.