43 Effect of Mushroom Probiotic (Coriolus Versicolor) on Galectin Gene Secretion in Goat Blood

Abstract

Abstract Galectins (GAL) constitute an evolutionarily conserved family of β-galactoside-binding proteins that are secreted. They are involved in the regulation of homeostasis, innate and adaptive immune responses to infectious challenge. The mushroom Coriolus versicolor (CV) has been reported to boost suppressed immune function, extending the survival rate and improving quality of life in man. The objective of this study was to evaluate the effect of mushroom probiotics on the secretion of Galectins in goat blood. Ten BoerXSpanish female goats (5 weeks of age) were used for this study. Powdered CV was soaked in cold water and sterile filtered. Following initial screening for infection, goats were assigned to two groups of five (n = 10). Goats were drenched daily with 10 mL cold (treatment I) extract for 4 weeks. A control group of five age-matched goats received sterile water (treatment II). Blood samples were collected on a weekly basis. The concentration plasma protein was determined by using Pierce BCA kit (Thermo Scientific Pierce, Rockford, IL). Galectins-1, -3, -8, -9 and -12 concentration was detected by using a commercial ELISA kits (ABclonal Biotechnology, Woburn, MA). There was an effect of treatment in total plasma protein concentration when compared to the control group (P < 0.02). Galectins tested were secreted in both control and treatment groups. Treatment with CV decreased the concentration of Gal-1, 8 and 9 and increased the concentration of Gal-3 and -12 (P < 0.05). Results from this study indicate that mushroom probiotics can modulate the secretion of GAL.