Abstract
This chapter discusses the chemical composition of proteins. The most important component of mitochondria is water. The bulk of the dry mass of mitochondria is composed of protein, the exact amount being determined by the proportion of membranes as they contain the second most common component of mitochondria, lipid. The proteins present in mitochondria consist of enzymes and the functionally related cytochromes and other carriers. A number of mitochondrial enzymes are soluble after extraction or disruption of the organelles and on the basis of their latency or otherwise, and the extent to which they can be extracted under progressively harsher conditions, are thought to be present in either the intracristal and/or peripheral spaces and the matrix space. More or less detailed information from biophysical and biochemical techniques including electron microscopy is available on the structure of some 30 or so of the mitochondrial enzymes. The chapter further discusses D-3-hydroxybutyrate dehydrogenase, L-3-hydroxyacyl CoA dehydrogenas, malate dehydrogenase, and isocitrate dehydrogenases.
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