Abstract
The binding of [ 3H]3,4-methylenedioxymethamphetamine ([ 3H]MDMA) to both rat brain membrane preparations and glass fiber filter papers was analyzed in the present study. Saturation studies indicate that [ 3H]MDMA binding is saturable and monophasic in both the presence and absence of rat brain membranes. This apparent ‘specific’ binding of [ 3H]MDMA in both the presence and absence of brain homogenates was totally eliminated by pretreating glass fiber filter papers with polyethylenimine. Drug competition studies demonstrated that [ 3H]MDMA binding displays a distinct ‘pharmacological’ profile in the absence of brain tissue. These data indicate that apparent [ 3H]MDMA binding to rat brain homogenates results from artifactual of [ 3H]MDMA to glass fiber filter paper. In addition, uptake of [ 3H]MDMA into rat brain synaptosomes could not be detected. We conclude the [ 3H]MDMA has limited usefulness in radioligand binding studies.
Published Version
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