Abstract
Background: VP1 structural protein plays a role as a key player in the pathogenesis, has a uniqueness that is interesting enough to be studied by studying the nature and function of structural proteins VP1. This study aims to predict the three-dimensional structure of proteins VP1 on EV-A71. Methods: The target protein is obtained from UniProt server with an access code A0A097EV89using templates 4cey.1.A (PDB ID) were analyzed in silico by homology method using SWISS-MODEL server. Results: Analysis showed the target protein and the template has 95.29% identity and is composed of 297 amino acids with a value of -2.15 QMEAN. Structural protein VP1 in Ramachandran Plots have a stable structure, non-glycine residue in the outlier regions only around 0.34% (A53 ALA) Rated rotamer outliers 1.61%. Conclusion: The three-dimensional structure model of the protein studied has a stable structure and the information obtained is useful for further research in developing vaccines for diseases caused by EV-A71.
Highlights
Protein struktural VP1 pada virus berperan penting dalam patogenesis, memiliki ciri khas sebagai pengendali utama entri seluler sel virus
The structural protein VP1 in a virus plays an important role in pathogenesis, has a characteristic as the main controller of cellular entry of viral cells
This study aims to predict the threedimensional structure of the VP1 protein on Enterovirus A71 (EV-A71)
Summary
Protein struktural VP1 (viral protein 1) pada virus berperan penting dalam patogenesis, memiliki ciri khas sebagai pengendali utama entri seluler sel virus. Penelitian ini bertujuan untuk memprediksi struktur tiga dimensi protein VP1 pada Enterovirus A71 (EV-A71). Hasil: Analisis penelitian menunjukkan protein target dan template memiliki identity 95,29 % dan tersusundari 297 asam amino dengan nilai QMEAN -2,15. Protein struktural VP1 pada Ramachandran Plots memiliki struktur stabil, residu non-glisin pada daerah outlier hanya berkisar 0,34 % (A53 ALA) dengan Nilai rotamer outliers 1,61 %.Kesimpulan: Model struktur tiga dimensi protein yang diteliti memiliki struktur stabil dan informasi yang didapatkan berguna untuk penelitian lebih lanjut dalam pengembangan vaksin penyakit yang disebabkan oleh EV-A71. Kata kunci: protein VP1; Enterovirus A71; Swiss-Model; Metode Homologi; In-Silico
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