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Abstract
A method based on mirror refection interference and fluorescence emission difference is provided to improve the three-dimensional resolution and SNR in confocal microscopy. Theoretical analysis and experimental results have been performed to verify the effectiveness of the method. Our numerical simulations predict that lateral resolution close to 0.285λ and axial resolution close to 0.205λ are possible for practical confocal microscopy with visible light. Furthermore, the SNR has also been improved. The experimental results of both fluorescent beads and cell microtubules are presented to verify the applicability and effectiveness of our method. The ability to increase the lateral resolution and axial resolution using mirror refection interference and fluorescence emission difference without increasing the laser power is of great importance for imaging fluorescent biological specimens, which cannot tolerate high laser power.
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