3D Printing of Cytocompatible Water-Based Light-Cured Polyurethane with Hyaluronic Acid for Cartilage Tissue Engineering Applications.

Ming-You Shie,Li-Ju Wei,Wen-Ching Chang,Yi-Wen Chen,Chien-Han Chen,Yu-Fang Shen,Yu-Hsin Huang,Cheng-Ting Shih

doi:10.3390/ma10020136

Abstract

Diseases in articular cartilages have affected millions of people globally. Although the biochemical and cellular composition of articular cartilages is relatively simple, there is a limitation in the self-repair ability of the cartilage. Therefore, developing strategies for cartilage repair is very important. Here, we report on a new liquid resin preparation process of water-based polyurethane based photosensitive materials with hyaluronic acid with application of the materials for 3D printed customized cartilage scaffolds. The scaffold has high cytocompatibility and is one that closely mimics the mechanical properties of articular cartilages. It is suitable for culturing human Wharton’s jelly mesenchymal stem cells (hWJMSCs) and the cells in this case showed an excellent chondrogenic differentiation capacity. We consider that the 3D printing hybrid scaffolds may have potential in customized tissue engineering and also facilitate the development of cartilage tissue engineering.

Highlights

Cartilage reconstruction is an important topic in regenerative medicine [1,2,3,4,5,6,7]
In order to substitute traditional solvent-based which uses light-cured volatile organic (VOC), water-based PU have in recent years [29]
We suggest thatadded the added could react with form strength in the in scaffolds with hyaluronic acid (HA)

Summary

Introduction

Cartilage reconstruction is an important topic in regenerative medicine [1,2,3,4,5,6,7]. Because of the avascularity of articular cartilage, the limited proliferation of mature chondrocytes and less migration of chondrocytes surrounded by the extracellular matrix, the regeneration of articular cartilage is considered difficult in comparison to other tissue [2]. Many approaches such as microfracture, abrasion arthroplasty, osteochondral autologous transfer, and autologous chondrocyte implantation have been applied for cartilage reconstruction. There is still no ideal and perfect approach for the reconstruction of critical articular cartilage defects and there are many problems that need to be resolved [8]

Methods

Results

Conclusion