3D printed self-calibrating on-site sensing platform based on bimodal excitation carbon dots for visual 4-nitrophenol detection by means of the localization of inner filter effect and pH regulation

Abstract

For facile and rapid detection of 4-nitrophenol (4-NP), we synthesize nitrogen-doped carbon dots (N-CDs) through microwave radiation treatment using melamine and o-phenylenediamine as precursors. We employed N-CDs as an ingenious ratiometric fluorescence sensor via a novel bimodal excitation behavior. As the concentration of N-CDs increases, the broad excitation peak splits into two excitation peaks. The blue-shift phenomenon appears at the short-wavelength excitation peak, while the long-wavelength excitation peak presents the red-shift. Consequently, 4-NP can be sensitively detected by N-CDs owing to the localization of inner filter effect (L-IFE) with the detection limit of 83 nM ranging from 0.083 to 80 µM. Moreover, a self-calibrating on-site sensing platform constructed by 3D printing technique realizes the visual 4-NP detection in actual samples based on bimodal excitation behavior and RGB analysis. Our work not only presents a novel perspective for visual 4-NP sensing, but also renders the integration of 3D printing technology and the CDs industry.