Abstract
Innovative drug screening platforms should improve the discovery of novel and personalized cancer treatment. Common models such as animals and 2D cell cultures lack the proper recapitulation of organ structure and environment. Thus, a new generation of platforms must consist of cell models that accurately mimic the cells’ microenvironment, along with flexibly prototyped cell handling structures that represent the human environment. Here, we adapted the 3D-bioprinting technology to develop multiple all-inclusive high throughputs and customized organ-on-a-chip-like platforms along with printed 3D-cell structures. Such platforms are potentially capable of performing 3D cell model analysis and cell-therapeutic response studies. We illustrated spherical and rectangular geometries of bio-printed 3D human colon cancer cell constructs. We also demonstrated the utility of directly 3D-bioprinting and rapidly prototyping of PDMS-based microfluidic cell handling arrays in different geometries. Besides, we successfully monitored the post-viability of the 3D-cell constructs for seven days. Furthermore, to mimic the human environment more closely, we integrated a 3D-bioprinted perfused drug screening microfluidics platform. Platform’s channels subject cell constructs to physiological fluid flow, while its concave well array hold and perfused 3D-cell constructs. The bio-applicability of PDMS-based arrays was also demonstrated by performing cancer cell-therapeutic response studies.
Highlights
Innovative drug screening platforms should improve the discovery of novel and personalized cancer treatment
We showed that we are able to take all our initial experiments to streamline the process of the generation of an inclusive organ-on-a-chip (OOC)-like device with the generation of 3D-Gelatin methacryloyl (GelMA) HCT116 culture model using our 3D-bioprinter
GelMA HCT 116 cell structures were printed into the wells and formed ring/toroidal shapes within a day after printing
Summary
Innovative drug screening platforms should improve the discovery of novel and personalized cancer treatment Common models such as animals and 2D cell cultures lack the proper recapitulation of organ structure and environment. In recent years several principles and technologies such as 3D-tissue and organ culture are merged with 3D-printing and microfluidic approaches to address the issues regarding the proper representation of cell models and to capture complex human physiology in vitro. One critical consideration is that OOC manufacturing demands flexibility and a broad resolution range in order to achieve the physiological relevance of the organ of interest Conventional fabrication techniques such as[25,26] lithography and laser micro-machining are limited by the necessity of complicated, expensive facilities, and e quipment[27]. An alternative option is 3D-bioprinted 3D-models, which have the potential to mimic cell viability, metabolic activity, and the vital reactions of organs or tissues
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