393 Helicobacter pylori Infection Causes Insulin Resistance Through the C-Jun/miR-203/SOCS3 Pathway

Abstract

of humanized rats (HR) collected 1, 2 and 30 days after inoculationanalyzed by 454sequencing and 16sRNA-qPCR. Fermentative activity was investigated by quantification of short chain fatty acids by chromatography and enzymatic reaction of D and L-lactate. HR were sacrificed at d30 after inoculation and compared to Conventional (CV) and GF rats. Ki67 and GLP-1 immunohistochemistry were performed on paraformaldehyde-fixed colon mucosa sections. Results are expressed as mean ± SEM and statistical analysed used non parametric tests. Results: The fecal sample from the SBS patient used as inoculum, contained high amount of Lactobacillus (1.5x1010bacteria/g of feces) and no C. Leptum consistent with our previous studies. Transfer of this lactobiota in GF rats reached a steady state consortium after 3 days. At that time, bacteria population shifted from enriched population of lactates producers to enriched population of lactates consumers. Although the lactobiota inoculum was modified in the environment of GF rats, the sequencing highlighted that HR microbiota conserved characteristics of the inoculum at the bacteria gender level (6.45x108 Lactobacillus bacteria/g of feces and lack C. Leptum). After inoculum transfer, the lactates production significantly dropped the first 3 days (from 155mM to undetectable levels) as propionate, and butyrate (from 5.34mM to 1.87mM and from 1.84mM to 0.55mM respectively) while acetate was not modify (form 11.62mM to 13.99mM). Analysis of HR colon mucosa, revealed an increase in Ki-67 proliferating cells in the crypt (+131%, p<0.05 vs GF) while no difference in crypt depth (HR vs GF) and a significant increase in the number of GLP1 positive cells (+50%, p<0.05 vs CV). Conclusion: These data indicate for the first time that microbiota from SBS patients can significantly affect the phenotype of colonic mucosa. They suggest that this lactobiota has an active role in the adaptative mechanisms following extensive small bowel resection.