Abstract
Many patients are admitted to the emergency department (ED) due to major trauma. These patients may suffer from massive hemorrhage, respiratory failure, and progress to hypovolemic shock state. It will lead to over-expression of inflammatory responses, malfunction of the immune system, homeostasis failure, and, eventually, multi-organ failure. Of the shock patient treatment, airway maintenance and oxygen supply are essential and of paramount importance. During the early stage of respiratory failure and shock state, the influence of hypoxia on a cellular level is not clearly known. This study aimed to investigate the effects of preventive measures (various medications) in a neuronal cell under hypoxia. The experiments were performed with SH-SY5Y cells. MTT viability assay, Cell apoptosis assay, Reactive oxygen species were done to measure the effectiveness of preventive medications, pentoxifylline (PTX), dexamethasone (DEXA), hypertonic saline (HTS).SH-SY5Y cells were treated with the above medication treatments before hypoxia (1% O2) and after hypoxia. Results:1. Apoptosis a. Pre-hypoxia: PTX, Dexa, and HTS treatments were effective in decreasing apoptosis. b. Post-hypoxia: Only Dexa was effective in decreasing apoptosis.2. ROS a. Pre-hypoxia: PTX, Dexa, and HTS treatments were effective in reducing ROS production. b. Post-hypoxia: Only Dexa was effective in decreasing ROS production.3. MTT viability None of the medications showed significant effectiveness. Our previous work showed PTX plays an essential role in minimizing damage to a neuronal cell under hypoxia. And we wanted to see if PTX and other medications may be useful in preventive measures. The experiment exhibited a possibility that PTX alone and with other medications may be effective preventive measures in shock patients.
Published Version
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