Abstract
The adsorption of nucleic acid bases (adenine, guanine, thymine, cytosine), their deoxy-nucleosides and deoxynucleotides has been investigated at a gold electrode by specular reflectivity measurements in 0.1 M NaClO 4. Reflectivity—potential curves of these compounds exhibited a decrease in reflectivity caused by their adsorption. All compounds are adsorbed in the potential range around the point of zero charge (p.z.c.). Adenine and guanine begin to be adsorbed at a more negative potential than thymine and cytosine, suggesting a larger adsorptivity of purine bases than pyrimidine. The adsorption of guanine and cytosine at high concentrations may accompany the reorientation and/or the dimer formation. Deoxynucleosides and deoxynucleotides, except deoxythymidine and dTMP, show a somewhat larger adsorptivity in the positive potential region than the corresponding bases, due to the participation of deoxyribose and phosphate groups. The results obtained from an analysis of the adsorption isother of adenine, thymine and their derivatives suggests that they are oriented with their base moieties flat to the negatively charged electrode surface.
Published Version
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