#375 : Sperm Quality and its Association with Embryonic Aneuploidy Outcomes in Preimplantation Genetic Testing for Aneuploidies (PGT-A)

Abstract

Background and Aims: The global prevalence of infertility is approximately 12% of couples worldwide. An epidemiological study by the World Health Organization (WHO) revealed the prevalence of infertility in low- and middle-income countries is 16.5%.1About 50% of the cases of infertility was found to be male infertility. Only one-third of patients that underwent IVF leads to clinical pregnancy. Sperm characteristics has been shown to contribute to IVF failure. The embryo developed through IVF may be aneuploid. The chromosomal composition can be examined using PGT-A.[Formula: see text] This study aims to determine the relationship between aneuploidy findings and poor-quality sperm, in terms of volume, concentration, morphology, motility, and DNA Fragmentation Index (DFI). Methods: This is a cross-sectional study. Sperm analysis data from 25 patients at Yasmin Fertility Clinic from 2020-2023 and 63 embryonic PGT-A results from the same patients obtained from Indonesian Reproductive Medicine Research and Training Center was included. Normality and correlation analysis was done using SPSS. Results: This analysis included data from 25 couples and 63 embryos. From the samples of embryo, about 66.67% (0.00-100.00%) were aneuploid with either monosomy, trisomy, or mosaic. The most common sperm abnormalities are oligoastenoteratozoospermia with 11 (17.46%) cases, followed by oligozoospermia (N = 7(11.11%)) and oligoteratozoospermia (N= 7(11.11%)). Infertility among 31 (49.2%) couples are caused by maternal condition. Sperm morphology is negatively correlated with aneuploidy percentage along withits types. Negative correlation can be seen in between sperm concentration and mosaicism, and between sperm DFI with number of aneuploid chromosome, monosomy, and mosaicism. However, the only statistically significant finding correlation is between sperm motility and mosaicism, which is correlated positively (r = 0.260, p = 0.043). Conclusion: A significant positive association was found between sperm motility and chromosomal mosaicism.