363 Probiotics Supplementation Increases Intestinal Blood Flow in Extremely Low Birth Weight (ELBW) Neonates

Abstract

Background: Proposed benefits of probiotics supplementation are multiple; recently, vasoactive properties of trace amines, produced by probiotics, have been shown to affect mesenteric vasculature in animal models. The effects of probiotics on intestinal blood flow have not yet been described. Aims: To determine whether probiotics supplementation affects intestinal blood flow velocity in ELBW neonates. Methods: In this randomized, double-blind, placebo-controlled study probiotics (500 M CFU of Lactobacillus rhamnosus and 500 million CFU of Bifidobacterium infantis) were added to the first enteral feeding and continued until discharge or 34 weeks postmenstrual age. Pulsed Doppler measured preprandial and postprandial (at 30 and 60 min) time-averaged mean, peak and end diastolic velocities (TAMV, PSV, EDV) during second week of life after 7 days of probiotics supplementation. Mean values across time were graphed for probiotics and control groups. The percent change from preprandial to 60 min post was compared by t-test, for each measurement method. Results: Thirty-one infants were studied, 15 randomized to probiotics and 16 to placebo. Postprandial TAMV were increased significantly in probiotics group (p = 0.048), with PSV and EDV showing a trend. There were no significant differences between groups in mean birth weight, gestational age, the time of the study, volume and type of feedings (p > 0.05). The percent of infants undergoing phototherapy, receiving caffeine or being diagnosed with patent ductus arteriosus was similar between groups (p > 0.05). Conclusions: Probiotics administration significantly increases intestinal blood flow in ELBW preterm neonates when compared to placebo group.