Abstract
Publisher Summary L-type calcium channels mediate long-lasting calcium currents in response to strong depolarizations, and are the principal calcium channels in muscle tissues. This chapter focuses on purification and reconstitution of the L-type calcium channel from skeletal muscle. They are distinguished by their sensitivity to organic calcium channel modulators including the dihydropyridines (DHP), phenylalkylamines, and benzothiazepines. In skeletal muscle, L-type calcium channels mediate a slowly activated, long-lasting calcium current in the transverse tubules, which is blocked by DHP calcium channel antagonists. L-type calcium channel activity is observed at the single-channel level after reconstitution of purified channel preparations into planar bilayers. The results of ion flux, patch pipette, and macroscopic bilayer methods show that purified skeletal muscle calcium channels retain selective ion conductance activity that is modulated appropriately by calcium channel-modulating drugs and protein phosphorylation.
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