Abstract
The adhesion of 32 Streptococcus pneumoniae isolates to human nasopharyngeal and buccal epithelial cells was investigated. Four strains, showing over 200 bacteria per nasopharyngeal cell were classified as highly adherent. Comparable results were obtained with buccal epithelial cells. The pneumococcal adhesin was shown to be proteinaceous. A novel adhesion assay was developed using epithelial cells immobilized in ELISA plates. This method enabled us to screen large numbers of isolates.
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