Abstract
A three-dimensional heteronuclear NMR technique to correlate 31P spins with 1H along the backbone of DNA is described. The approach is based on a 31P–1H heteronuclear long-range correlation (HELCO) experiment in its refocused form. The utility of this technique is demonstrated by the sequence-specific resonance assignment of 31P and 1H spins along the backbone of a mismatched base-paired dodecanucleotide, d-GGTACIAGTACC. Copyright © 2000 John Wiley & Sons, Ltd.
Published Version
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