31. PHARMACOLOGICAL PREVENTION OF ISCHEMIA REPERFUSION INJURY IN RATS SUBJECTED TO LOWER LIMB ISCHEMIA

Abstract

Abstract Introduction The use of tourniquet to achieve a blood-free surgical field is a common practice in orthopedic surgery. Blood flow restoration may lead to systemic inflammatory and oxidative stress damage known as ischemia/reperfusion injury (IRI). The aim of this work is to evaluate whether the administration of dexketoprofen (DEX) before reperfusion contributes to the prevention of IRI-related damage. Methods Male WAG/RijHsd rats were divided into 3 groups: two of them underwent 3h of ischemia and were administered either dexketoprofen (1 mg/kg) or vehicle; the third group was used as control, not subjected to ischemia/reperfusion. The treatment was given orally 30 minutes before reperfusion. Systemic affectation was evaluated 3h, 24h and 14 days after ischemia; at the anatomopathological level, limb edema was measured after 24h; finally, functional recovery was evaluated during the 14 days following ischemia by the rotarod and treadmill tests. Results IRI-related hepatic and muscular damage was significantly reduced by dexketoprofen, although its effect on hepatic damage was seen earlier. However, the treatment had no beneficial effect on renal damage, nor did it reduce limb edema with respect to the vehicle group (63±7.8 vs 73±10mm; p>0.05). In addition, dexketoprofen anticipated functional recovery between 2 and 9 days compared to untreated animals, as assessed by rotarod and treadmill tests. Conclusion Dexketoprofen treatment proved to be effective, reducing both hepatic and muscular damage secondary to IRI, as well as hastening the functional recovery of treated animals.