Abstract
The aim of this paper was to find a methodology to identify the specific biochemical characteristics of gastric malignant tissues in different stages using 2DIR correlation spectroscopy and chemometric methods. For this, a group of 15 patients in stage 3 and 4a of gastric cancer evolution were used as subjects. From every patient, 5 pairs of normal and malignant tissues were collected and investigated just after surgical rejection, by infrared spectroscopy (IR), principal component analysis (PCA) and two dimensional IR correlation spectroscopy (2D-COS). The spectral differences could be correlated with the modified molecular structure of proteins and lipids from the two tissue types, and with the variation in their glycogen content. A discrimination between the stage of the cancer evolution was not possible through simple IR spectroscopy, therefore PCA analysis was used. However, PCA alone did not give a clear discrimination between normal and malignant tissue, because the scores of the normal and cancer samples were partial overlapped. By 2D-COS we were able to obtain additional information about the structural changes. Although, synchronous spectra look similar for different stages of cancer evolution, the asynchronous spectra allowed a clear distinction between them. Moreover, using the Noda’s rules, the sequential order of bands evolution was obtained. Following the results we can conclude that this technique may be suitable as a screening method to discriminate the stage of the cancer evolution and also for establishing the sequences of the variation of different structural entities.
Published Version
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