Abstract
Immunological resistance against Friend virus seems to be primarily mediated vy antibody formation. No cell-mediated immune response against the virus was found in immunized mice. Besides immunological basis. Other mechanisms play a major role in resisteance and susceptibility. The possibility of a ’target cell“ capable of virus binding in the hematopoietic tissues of susceptible mice was studied by using a new tecnique. Known numbers of focus units (FFU) of Friend virus B (passaged in DBA 2 mice) were incubated with variable amounts of cells from different origins. After 30 min of incubation, the supernatant was injected intravenously in DBA/2 susceptible mice. Nine days later, the spleens were removed and the number of surface foci was counted after immersion in Bouin's fixative. The number of foci observed in these animals represents the number of FFU left in the supernatant after incubation. Controls were injected with unabsorbed virus kept for 30 min at room temperature. Using this method, we found that the Friend virus was adsorbed by spleen, bone marrow, or thymus cells of mice from susceptible strains, e.g., C31I or DBA/2, but was not adsorbed by other tissues from the same strains. By contrast, when spleen or bone marrow cells from resistant strains were incubated, e.g., C57BL/I, C57BL/6, C58, the virus was not adsorbed and the original number of FFU was obtained in the supernatant. These date strongly suggest the presence of a target cell in hematopoietic tissues of susceptible mice and its absence in resistean strains.
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