267 Generation and Characterization of a CRISPR-Cas9-Mediated XPC gene Knockout in Human Skin Cells

Abstract

Xeroderma pigmentosum group C (XPC) is a multifunctional DNA damage recognition protein involved in the global genome nucleotide excision repair (GG-NER) pathway. Mammalian cells employ this pathway to eliminate bulky DNA lesions that induce double helix distortion caused by mutagens such as UV radiation or cisplatin. Loss-of-function mutations in the XPC gene lead to various malignancies, including skin cancers. Photosensitivity and the buildup of DNA damage can both define the phenotype of XPC patients’ cells.