Abstract
The homeostasis and the functions of the skin also depend on the stable organization and cohesion between the epidermis and the dermis, the two main compartments of the skin. The dermo-epidermal junction (DEJ), a highly complex structure allows this interconnection between the epidermis and the dermis. The DEJ is a highly specialized structure playing essential roles in different processes as cell adhesion, differentiation, proliferation, survival, motility, in the signal transmission and in the formation of the barrier permeability. In the present study, we used bioinformatics to develop a proprietary bioinformatic network model to understand interactions between genes and components involved in dermo-epidermal junction. Through this bioinformatics model we would like to understand the importance of the DEJ, in the maintain of the skin functions and structure. Then we developed several in vitro models to study the role of this DEJ. Our full thickness model including a dermis (fibroblasts included in collagen matrix) and an epidermis above allows to study the expression of DEJ proteins. These bioengineered full thickness model allows to study the communication between cells inside the dermal compartment and the intercommunication between dermis and epidermis. Characterization of the cellular phenotype by microscopic observation, expression of proteic markers and qPCR was undertaken in order to point out specific features according to the gene silenced. This complementary in silico and in vitro testing approach will facilitate the study of the modulating potential of biofunctional ingredient.
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