25(OH)D AS A RISK FACTOR OF ESSENTIAL ARTERIAL HYPERTENSION CONSIDERING THE GENES POLYMORPHISM AGTR1 (RS5186) AND VDR (RS2228570)

Abstract

Objective: Essential arterial hypertension (EAH) remains the most common global non-communicable disease. More than 50% of patients with hypertension have additional cardiovascular risk factors. The aim of this study is to establish the role of 25-hydroxyvitamin D as an additional risk factor of EAH depending on 1166A>C polymorphism of AGTR1 gene (rs5186) and A/G polymorphism of VDR gene (rs2228570). Design and method: 100 subjects with EAH and target-organ damaging (2nd stage), moderate, high, very high cardiovascular risk were involved in the case-control study. Among them, 70.84% females, 29.16% males, mean age 57.86 ± 7.81 yo. The control group consisted of 60 healthy individuals of relevant gender and age. All recruited subjects were tested for serum levels 25-hydroxyvitamin D (immune luminescent test “MAGLUMI’’, “SNIB’’, China). To detect gene polymorphism the qualitative real-time polymerase chain reaction was done. AGTR1 gene genotyping was performed for 72 patients and 48 healthy individuals and VDR gene – for 100 patients and 60 healthy subjects. Results: EAH associates with the decreased 25-hydroxyvitamin D (<30 ng/ml) regardless the genotypes AGTR1 (rs5186) and VDR (rs2228570) genes. Reduced serum level of 25-hydroxyvitamin D escalate the risk of EAH almost threefold (OR – 2.90; 95%CI OR:1.0–8.59; p = 0.048). Conclusions: Low level of 25-hydroxyvitamin D increases the EAH risk regardless the AGTR1 (rs5186) and VDR (rs2228570) genes’ alleles in the examined population.