Abstract
Vitamin D has been proposed to have extraskeletal actions in several chronic diseases, including some cancers. The role of vitamin D in non-melanoma skin cancer (NMSC), the most prevalent cancer in the US, is debated. The objective of this work was to investigate the effects of dietary vitamin D3 in an animal model of NMSC. Equal numbers of male and female SKH1 hairless and immunocompetent mice (n = 150) were fed 25, 150, or 1000 IU vitamin D3 for 29 weeks. Subgroups were exposed to one minimal erythemal dose of UVB light 3x/week for the last 25 weeks of the study. Vitamin D metabolites, 25-hydroxyvitamin D (25OHD) and C-3epi-25-hydroxyvitamin D (C3epi), were quantified in skin and serum from a representative sampling of mice (n = 36) using isotope dilution high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Levels of 25OHD rose in a dose dependent manner in serum and skin (P < 0.01), ranging from 4.5-35.8 nmol/L and 2.6-11.7 pmol/g, respectively. Serum and skin C3epi also increa...
Published Version
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