242 - Antioxidant and Cytoprotective Activity in Vitro of Aqueous Extract of Three Morphotypes of Solanum Spp. "native Potato"

Abstract

Introduction The native potato grows to greater heights of 3000 meters, in addition to its high energy and nutritional value is a source of secondary metabolites with antioxidant activity that could help in the prevention of diseases associated with oxidative stress. The aim of this study was to evaluate the antioxidant and cytoprotective activity in vitro of three morphotypes aqueous extract of Solanum spp (native potato) from the Instituto Nacional de Innovacion Agraria (INIA) - Ayacucho, Peru. Materials and methods Aqueous extracts of the three samples were prepared. It was measured antioxidant capacity, contents of polyphenols and flavonoids and the cytoprotection capacity in vitro. The antioxidant capacity was determined by scavenging of DPPH● and ABTS●+ radicals. Polyphenols were determined with the Folin - Ciocalteu and flavonoids with AlCl3. Cytoprotection capacity was evaluated in rat liver (Holtzman) homogenate after lipoperoxidation induction with the system Fe2+/ascorbate; lipid peroxidation was measured by the spectrophotometric technique of thiobarbituric acid reactive species (TBARS). Results The results of antioxidant capacity with DPPH●. radical expressed as IC50 were between 387.6 to 459 μg/mL and TEAC-DPPH● between 2.2 to 2.60 mg/mg soluble matter; for ABTS●+, IC50 were between 186.1 to 292.2 μg/mL and TEAC-ABTS●+ between 9.3 to 14.6 mg/mg s.m. The total polyphenol content was in the range of 11.67 to 22.94 EAG μg/mg s.m.; and flavonoids between 4.58 to 6.64 μg EQD/mg s.m. The result of cytoprotection with TBARS test showed that lipid peroxidation was decreased significantly by extracts (p˂0,005). Conclusion The aqueous extract of the three morphotypes native potato presented cytoprotective effect and antioxidant activity in vitro, and constitute potential source of antioxidants for prevention of diseases associated with oxidative stress.