2199 RECELLULARIZATION OF WHOLE ORGAN MATRIX FOR RENAL TRANSPLANTATION

Abstract

You have accessJournal of UrologyTransplantation & Vascular Surgery: Renal Transplantation, Vascular Surgery1 Apr 20112199 RECELLULARIZATION OF WHOLE ORGAN MATRIX FOR RENAL TRANSPLANTATION Sayed Hadi Mirmalek Sani, David Sullivan, Giuseppe Orlando, Sigrid Agcaoili, Juan Arenas Herrera, Shay Soker, Tamer Aboushwareb, James Yoo, and Anthony Atala Sayed Hadi Mirmalek SaniSayed Hadi Mirmalek Sani Winston Salem, NC More articles by this author , David SullivanDavid Sullivan Winston Salem, NC More articles by this author , Giuseppe OrlandoGiuseppe Orlando Winston Salem, NC More articles by this author , Sigrid AgcaoiliSigrid Agcaoili Winston Salem, NC More articles by this author , Juan Arenas HerreraJuan Arenas Herrera Winston Salem, NC More articles by this author , Shay SokerShay Soker Winston Salem, NC More articles by this author , Tamer AboushwarebTamer Aboushwareb Winston Salem, NC More articles by this author , James YooJames Yoo Winston Salem, NC More articles by this author , and Anthony AtalaAnthony Atala Winston Salem, NC More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.2438AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Although transplantation has become the standard treatment for renal failure, the demand for transplantable organs has markedly surpassed the supply. Cell based therapies using regenerative medicine techniques have emerged as an alternative method to organ transplantation. We have recently developed a novel method of generating acellular whole organ matrix as a scaffold for renal cell transplantation. In this study we investigated whether cells could be effectively distributed throughout the acellular kidney scaffold with the long-term goal of bioengineering a transplantable kidney. METHODS Porcine kidneys were obtained and resident cells were removed by perfusing the renal arteries with detergent and rinsing solutions. Acellular kidney scaffolds were statically seeded with murine MS1 endothelial cells and analyzed with MTS cell proliferation assay for biocompatibility/toxicity studies. Primary renal cells were seeded into the renal parenchyma, and MS1 cells were perfused through the renal artery. The whole organ scaffold was maintained in a perfusion bioreactor up to 72hrs for morphological analysis. RESULTS Whole porcine kidneys were successfully decellularized (removal of cells) within 7 days. Complete infiltration of media via a perfusion bioreactor confirmed the maintenance an intact vascular network. MS1 cells seeded on the scaffolds remained viable under culture conditions, indicating the biocompatibility of the scaffold. Cell attachment was observed with both injection and perfusion seeding of renal and endothelial cells, respectively, however, cell aggregation was observed in smaller vessels at all segments of the kidney. CONCLUSIONS These studies demonstrate that the use of acellular whole organ matrix as a scaffold for cell transplantation is feasible. The processed scaffolds are biocompatible and renal cells can be distributed throughout the scaffolds using optimized protocols. This technique provides a significant platform for the bioengineering of whole organs and may become an alternative method to transplantation. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e882 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Sayed Hadi Mirmalek Sani Winston Salem, NC More articles by this author David Sullivan Winston Salem, NC More articles by this author Giuseppe Orlando Winston Salem, NC More articles by this author Sigrid Agcaoili Winston Salem, NC More articles by this author Juan Arenas Herrera Winston Salem, NC More articles by this author Shay Soker Winston Salem, NC More articles by this author Tamer Aboushwareb Winston Salem, NC More articles by this author James Yoo Winston Salem, NC More articles by this author Anthony Atala Winston Salem, NC More articles by this author Expand All Advertisement Advertisement Loading ...